Product Description

NRF-1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7179-50, BS7179-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16656

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IF

All Applications: WB 1:500 - 1:2000
IF 1:50 - 1:200

Background: Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells. The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis. In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology. NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis. NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NRF-1 polyclonal antibody detects endogenous levels of NRF-1 protein.

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of NRF-1 polyclonal antibody

Alternative Name: Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

Immunogen: Recombinant full length Human NRF-1.

Conjugate: Unconjugated

Modification: Unmodified