NRF2 (Phospho-S40) Rabbit Monoclonal Antibody-MB66296

NRF2 (Phospho-S40) Rabbit Monoclonal Antibody

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Sizes: 50µl, 100µl

Catalogue Numbers: MB66296-50, MB66296-100

Swiss-Prot: Q16236

Host: Rabbit

NRF2 (Phospho-S40) Rabbit Monoclonal Antibody-MB66296

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/100)

Background: The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation. Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes. The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of NRF2 (pS40) protein.

Bioworld Molecular Weight: ~ 94 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NRF2 (pS40) expression in K562 (A), Hela (B) whole cellLysates., Immunohistochemical analysis of NRF2 (pS40) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.116) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor erythroid derived 2 like 2

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser40 of human Nrf2

Modification: Phosphorylation

Conjugate: Unconjugated