Product Description

NSE Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90971-50, BS90971-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09104(Human) P17183(Mouse) P07323(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, zebrafish

Applications: WB, ICC IHC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, α Enolase, β Enolase and γ Enolase. α Enolase expression has been detected on most tissues, whereas β Enolase is expressed predominantly in muscle tissue and γ Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NSE polyclonal antibody detects endogenous levels of NSE protein.

Bioworld Molecular Weight: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NSE on different lysates using anti-NSE antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: 293, ICC staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NSE, ENO2, 2 phospho D glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, Eno 2, ENO2, ENOG, ENOG, Enolase 2 (gamma, neuronal), Enolase 2, Enolase 2 gamma neuronal, Enolase2, Epididymis secretory protein Li 279, Gamma enolase, Gamma-enolase, HEL S 279, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neuron-specific enolase, Neurone specific enolase, NSE,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified