Product Description

NSE Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ17028-50, BZ17028-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P09104

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

Background: ENO2 an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 47 kDa; Observed MW: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NSE in mouse brain, rat brain lysates using NSE antibody., Western blot analysis of Enolase2/NSE in Human Fetal Brain lysates using Enolase2/NSE antibody., Immunocytochemistry analysis of NSE in Hela using NSE antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using NSE antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

Immunogen: A synthetic peptide of human NSE

Conjugate: Unconjugated

Modification: Unmodified