NTR1 (H218) Polyclonal Antibody-BS2820

NTR1 (H218) Polyclonal Antibody

Catalogue Numbers: BS2820-50, BS2820-100

Sizes: 50µl, 100µl

Alternative Name: NTSR1; NT-R-1; NTR; NTRH; NTRR

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30989

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Neurotensin (NT) initiates an intracellular response by interacting with the G protein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor, levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3 receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and in peripheral tissues where NT receptors can contribute to hypotension, hyperglycemia, hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60 cells express NTR1, which can couple to Gq, Gi/o, or Gs. Alternative splicing of rat NTR2 can generate a 5-transmembrane domain variant isoform that is co-expressed with the full-length NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglial cell line N11 induces MIP-2, MCP-1, IL-1β and TNFα in an ERK1/2 and Akt kinase-dependent manner.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NTR1 (H218) polyclonal antibody detects endogenous levels of NTR1 protein.

Bioworld Molecular Weight: ~36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NTR1 (H218) polyclonal antibody in extracts from hela cells.

Immunogen: Synthetic peptide, corresponding to amino acids 190-240 of Human NTR1.

Conjugate: Unconjugated

Modification: Unmodified