Product Description

Nucleophosmin Monoclonal Antibody

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB66818-50, MB66818-100

Sizes: 50µl, 100µl

Swiss-Prot: P06748

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: The transport of proteins across the nuclear envelope is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex, and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. For example, two cytosolic factors critically involved in the recognition and docking process are the karyopherin a and karyopherin b proteins. The karyopherin holoenzyme is a heterodimer of a and b subunits. The nuclear protein B23 (also referred to as nucleophosmin) is involved in ribosomal assembly and rRNA transport. B23 is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Nucleophosmin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Nucleophosmin expression in Hela (A), Jurkat (B), MCF7 (C), NIH3T3 (D) whole cell lysates., Immunohistochemical analysis of Nucleophosmin staining in human thymus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 37 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

Immunogen: Recombinant fusion protein of human Nucleophosmin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified