Nuf2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90975-50, BS90975-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9BZD4(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, FC
All Applications: WB:1:500-1:2,000
FC:1:50-1:100
Background: This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Nuf2 polyclonal antibody detects endogenous levels of Nuf2 protein.
Bioworld Molecular Weight: 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution.
Positive control:
Line 1: K562
Line 2: PC-3M
Line 3: THP-1, Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Alternative Name: hNuf2, hNuf2R, hNuf2R, Cancer/testis antigen 106, CDCA 1, CDCA1, Cell division cycle associated 1, Cell division cycle associated protein 1, Cell division cycle-associated protein 1, CT106, hNuf 2, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, Nuf 2, NUF2, NUF2 NDC80 kinetochore complex component, NUF2 NDC80 kinetochore complex component homolog, NUF2, S. cerevisiae, homolog of, NUF2, NUF2R,
Immunogen: Recombinant protein within human Nuf2 aa 1-200.
Conjugate: Unconjugated
Modification: Unmodified