NUP153 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90977-50, BS90977-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P49790(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF FC
All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
FC:1:50-1:100
Background: Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup153 is a peripheral NPC component that is implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Nup153 contains a unique N-terminal region, a central domain consisting of four to five zinc fingers and a C-terminal region containing about 30 irregularly spaced FXFG repeats. Nup153 is cleaved by caspases during apoptosis. Nup153 interacts wtih TAP, which is essential for mRNA export, and associates with chromatin towards the end of anaphase, in parallel with the inner nuclear membrane protein LAP2. Nup153 is involved in NPC assembly, in anchoring NPCs within the nuclear envelope and in mediating specific nuclear import events.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: NUP153 polyclonal antibody detects endogenous levels of NUP153 protein.
Bioworld Molecular Weight: 154 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of NUP153 on K562 (1) and HepG2 (2) cell lysate using anti-NUP153 antibody at 1/500 dilution., ICC staining NUP153 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: NUP153, 153 kDa nucleoporin, HNUP153, N153, NU153, Nuclear pore complex protein hnup153, Nuclear pore complex protein Nup153, Nucleoporin 153kDa, Nucleoporin Nup153, Nup 153, Nup153,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified