Product Description

NUP98 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0111-50, MB0111-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P52948

Host: Mouse

Reactivity: Human

Applications: WB, IP,

All Applications: WB: 1:1000
IP: 1:50~1:200

Background: Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of NUP98 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 98kDa
Observed band size: 98kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of NUP98 in Hela, Jurkat and MCF7 cell lysates using NUP98 antibody (1:1000 diluted)., Immunoprecipitation analysis of Hela cell lysates using NUP98 antibody

Alternative Name: Nuclear pore complex protein Nup98; 98 kDa nucleoporin; Nucleoporin Nup98; Nuclear pore complex protein Nup96; 96 kDa nucleoporin;
Nucleoporin Nup96; ADIR2; NUP96; NUP196

Immunogen: Recombinant full length Human NUP98.

Conjugate: Unconjugated

Modification: Unmodified