Product Description

Nur77/NR4A1 (G347) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS3260-50, BS3260-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimμlation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: Nuclear receptor subfamily 4 group A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Nur77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicμlar receptor 3, NR4A1, GFRP1, HMR, NAK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 320-365 of Human Nur77/NR4A1.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Nur77/NR4A1 (G347) polyclonal antibody detects endogenous levels of Nur77 protein.

Molecular Weight: ~ 60 kDa

Pathway: Nuclear Receptor Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P22736

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only