NURR1 Polyclonal Antibody-GCP54

NURR1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: GCP54-50, GCP54-100

Sizes: 50µl, 100µl

Swiss-Prot: P43354

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encodeorphan nuclear receptors which may comprise an additional subfamily withinthe nuclear receptor superfamily. The rat and human homologs of mouse Nurr1are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growthfactor inducible, immediate early response genes. Induction of both Nurr1 andNur77 is seen after membrane depolarization while only Nur77 induction isseen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nurr77 expression is seen in activated T cells during G0 to G1 transition andthroughout the G1 phase. In addition to its function as an immediate earlygene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, apotent immuno-suppressant, has been shown to inhibit the ability of Nur77to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirementof Nur77 for TCR-mediated apoptosis is still under debate.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NURR1 polyclonal antibody detects endogenous levels of NURR1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NURR1 polyclonal antibody at 1:2000 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Human Brain using NURR1 antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Nuclear receptor subfamily 4 group A member 2; Immediate-early response protein NOT; Orphan nuclear receptor NURR1; Transcriptionally-inducible nuclear receptor; NR4A2; NOT; NURR1; TINUR

Immunogen: Synthetic peptide, corresponding to Human NURR1.

Conjugate: Unconjugated

Modification: Unmodified