Product Description

OAT Polyclonal Antibody

Catalogue Numbers: BS5833-50, BS5833-100

Sizes: 50µl, 100µl

Alternative Name: Ornithine aminotransferase, mitochondrial; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase; Ornithine aminotransferase, hepatic form; Ornithine aminotransferase, renal form

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04181

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: OAT (ornithine aminotransferase (mitochondrial), ornithine-oxo-acid aminotransferase) is a 439 amino acid protein encoded by the human gene OAT. OAT belongs to the class III pyridoxal-phosphate-dependent aminotransferase family and is usually found as a homotetramer in the mitochondrion matrix. OAT catalyzes the major catalytic reaction for ornithine. Ornithinemia, presumably due to deficiency of ornithine ketoacid aminotransferase (OAT) has been found in patients with gyrate atrophy of the choroid and retina. The clinical history of gyrate atrophy is usually night blindness that begins in late childhood, accompanied by sharply demarcated circular areas of chorioretinal atrophy. During the second and third decades the areas of atrophy enlarge. The hepatic cleavage product, hepatic OAT, is formed by cleaving a 25 amino acid transit peptide from the N-terminus of the OAT precursor. The renal form is produced by cleaving a 35 amino acid transit peptide from the N-terminus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: OAT polyclonal antibody detects endogenous levels of Ornithine aminotransferase (mitochondrial) protein.

Bioworld Molecular Weight: ~ 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of OAT polyclonal antibody at 1:500 dilution
Lane 1: A549 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 171-216 of Human OAT.

Conjugate: Unconjugated

Modification: Unmodified