Product Description

Ob-R (L662) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS3460-50, BS3460-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob resμlts in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postμlated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regμlate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Leptin receptor, LEP-R, HuB219 OB receptor, OB-R, CD295, LEPR, DB, OBR

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 650-700 of Human Ob-R.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ob-R (L662) polyclonal antibody detects endogenous levels of Ob-R protein.

Molecular Weight: ~ 132 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P48357

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only