Product Description

Ob-R (L662) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3460-50, BS3460-100

Sizes: 50µl, 100µl

Swiss-Prot: P48357

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ob-R (L662) polyclonal antibody detects endogenous levels of Ob-R protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Ob-R (L662) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: SK-OVCAR3 whole cell lysate (40ug)
Lane 4: L02 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Ob-R (L662) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 132 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Leptin receptor; LEP-R; HuB219 OB receptor; OB-R; CD295; LEPR; DB; OBR

Immunogen: Synthetic peptide, corresponding to amino acids 650-700 of Human Ob-R.

Conjugate: Unconjugated

Modification: Unmodified