Product Description

Occludin (A23) Polyclonal Antibody

Catalogue Numbers: AP0765-50, AP0765-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16625

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by polyunsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Occludin (A23) polyclonal antibody detects endogenous levels of Occludin protein.

Extra Notes: Western blot (WB) analysis of Occludin (A23) polyclonal antibody at 1:1000 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:The Kidney tissue lysate of Mouse(30ug)
Lane4:The Kidney tissue lysate of Rat(30ug)

Bioworld Molecular Weight: ~ 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: OCLN

Immunogen: A synthetic peptide corresponding to residues in Human Occludin.

Conjugate: Unconjugated

Modification: Unmodified