OCT1 (Phospho-S385) Polyclonal Antibody-BS67588

OCT1 (Phospho-S385) Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Sizes: 50µl, 100µl

Catalogue Numbers: BS67588-50, BS67588-100

Swiss-Prot: P14859; P09086

Host: Rabbit

OCT1 (Phospho-S385) Polyclonal Antibody-BS67588

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background: Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain . Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc , OBF-1 (a B-cell transcriptional coactivator protein) , TFIIB , and TBP (TATA-box-binding protein) . Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of OCT1 with a phosphorylation site at S385 protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of OCT1 (pS385) expression in mouse lung (A), rat lung (B) whole cellLysates., Immunohistochemical analysis of OCT1 (pS385) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: POU2F1; OCT1; OTF1; POU domain class 2 transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1; POU2F2; OCT2; OTF2; POU domain class 2 transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human OCT1 with a phosphorylation site at S385. The exact sequence is proprietary.

Modification: Phosphorylation

Conjugate: Unconjugated