ODF2 Polyclonal Antibody-BS76403

ODF2 Polyclonal Antibody Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Sizes: 50µl, 100µl Catalogue Numbers: BS76403-50, BS76403-100 Swiss-Prot: Q5BJF6 Host: Rabbit ODF2 Polyclonal Antibody-BS76403 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as ' Cenexins' , encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Unmodification Bioworld Molecular Weight: 80kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using ODF2 antibody at dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s., Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: ODF2; CT134; ODF2/1; ODF2/2; ODF84 Immunogen: Recombinant fusion protein of human ODF2(NP_702911.1). Modification: Unmodified Conjugate: Unconjugated