Omsk Hemorrhagic Fever Virus E Protein Ectodomain Polyclonal Antibody - NCP0151P

Omsk Hemorrhagic Fever Virus E Protein Ectodomain Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0151P-50, NCP0151P-100

Swiss-Prot: NC_005062.1

Host: Rabbit

Reactivity: OHFV-E

Applications: WB, ELISA

All Applications: WB: 1:500~1:2000
ELISA: 1:5000~10000

Background: Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion (OHFV-ΔNS1) and reporter virus replacing NS1 with the Gaussia luciferase (Gluc) (OHFV-ΔNS1-Gluc). Both the defective OHFV-ΔNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1 (BHK21NS1) but not in naïve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z' value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Omsk hemorrhagic fever virus E protein ectodomain polyclonal antibody detects endogenous levels of OHFV-E protein.

Note: For research use only, not for use in diagnostic procedure.

Immunogen: Recombinant protein of OHFV-E.

Conjugate: Unconjugated

Modification: Unmodified