OPN (L285) Polyclonal Antibody-BS1264

OPN (L285) Polyclonal Antibody

Catalogue Numbers: BS1264-50, BS1264-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10451

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: OPN (L285) polyclonal antibody detects endogenous levels of OPN protein.

Extra Notes: Western blot (WB) analysis of OPN (L285) polyclonal antibody at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:AML-12 whole cell lysate(40ug)
Lane3:L02 whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 35, 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; Nephropontin; OPN; Osteopontin; OSTP; Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; Urinary stone protein; Uropontin;

Immunogen: Synthetic peptide, corresponding to amino acids 251-300 of Human OPN.

Conjugate: Unconjugated

Modification: Unmodified