Osteoprotegerin Monoclonal Antibody-MB9396

Osteoprotegerin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9396-50, MB9396-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00300(Human) O08712(Mouse) O08727(Rat)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Bone morphogenesis and remodeling involve the formation of bone from osteoblasts and the resorption of bone by osteoclasts. The cytokine osteoprotegerin (OPG), also designated osteoclastogenesis inhibitory factor (OCIF), is known to inhibit osteoclast formation. A secreted glycoprotein, OPG is a member of the TNF receptor family that increases bone density and volume. OPG is thought to inhibit osteoclastogenesis by disrupting the cell-to-cell signaling between osteoblastic stromal cells and osteoclast progenitors. OPG is known to bind to TRAIL, a death domain-containing protein, and to inhibit TRAIL apoptosis in Jurkat cells. OPG also binds to osteoclast differentiation factor (ODF), also known as TRANCE/RANKL, a membrane-bound protein belonging to the TNF ligand family. Both TNFα and TNFβ upregulate OPG expression, while the bone resorbing agent prostaglandin E2 downregulates OPG.

Purification and Purity: ProG purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Osteoprotegerin monoclonal antibody detects endogenous levels of Osteoprotegerin protein.

Bioworld Molecular Weight: 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/1000 dilution., ICC staining Osteoproteger in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TNFRSF11B, OCIF, OPG, MGC29565, OCIF, OPG, Osteoclastogenesis inhibitory factor, Osteoprotegerin, PDB5, TNF receptor superfamily member 11b, TNFRSF 11B, TNFRSF11B, TR 1, TR1, TR11B, Tumor necrosis factor receptor superfamily member 11B,

Immunogen: Recombinant protein within human Osteoprotegerin aa 1-410.

Conjugate: Unconjugated

Modification: Unmodified