Product Description

OTC Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71341-50, BS71341-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00480

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:1000 - 1:4000

Background: This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using OTC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 38kDa

Specificity: Polyclonal Antibodies

Alternative Name: OTC; OCTD

Immunogen: Recombinant fusion protein of human OTC(NP_000522.3).

Conjugate: Unconjugated

Modification: Unmodified