P-glycoProtein 1 (I583) Polyclonal Antibody-BS3523

P-glycoProtein 1 (I583) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3523-50, BS3523-100

Sizes: 50µl, 100µl

Swiss-Prot: P08183

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The MDR proteins (Mdrs) are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr functions as an energy-dependent efflux pump for structurally diverse agents ranging from ions to peptides. It is implicated in the development of the multiple drug resistance observed in human cancer cells following prolonged chemotherapy. The classic form of MDR is associated with an increase in the Mdr protein, but not all cases of MDR can be attributed to a rise in Mdr levels. Mdr-1 is an apical transmembrane protein that is an integral part of the blood-brain barrier and functions as a drug-transport pump transporting a variety of drugs from the brain back into the blood. In the human population, there are 15 polymorphisms in the Mdr-1 gene.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: P-glycoprotein 1 (I583) polyclonal antibody detects endogenous levels of P-glycoprotein 1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of P-glycoprotein 1 (I583) polyclonal antibody at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: HCT116 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of P-glycoprotein 1 (I583) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 180 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD243; ABCB1; MDR1; PGY1; P-gp

Immunogen: Synthetic peptide, corresponding to amino acids 551-600 of Human Mdr-1.

Conjugate: Unconjugated

Modification: Unmodified