Product Description

p116 Rip Polyclonal Antibody

Catalogue Numbers: BS62404-50, BS62404-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q6WCQ1

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p116 Rip polyclonal antibody detects endogenous levels of p116 Rip protein.

Extra Notes: Western blot (WB) analysis of p116 Rip polyclonal antibody at 1:500 dilution
LaneA:PC12 whole cell lysate
LaneB:MEF whole cell lysate
LaneC:A549 whole cell lysate
LaneD:A2780 whole cell lysate

Bioworld Molecular Weight: ~ 116 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP

Immunogen: Synthetic peptide, corresponding to Human p116 Rip.

Conjugate: Unconjugated

Modification: Unmodified