Product Description

p150 CAF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90996-50, BS90996-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13111(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IF, IP, IHC FC

All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50
FC:1:50-1:100

Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated Histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: p150 CAF1 polyclonal antibody detects endogenous levels of p150 CAF1 protein.

Bioworld Molecular Weight: 107 kDa (Predicted band size)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution., ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CAF-1 subunit A, CAF-I 150 kDa subunit, CAF-I p150, hp150, CAF 1, CAF 1 subunit A, CAF, CAF I 150 kDa subunit, CAF I p150, CAF-1 subunit A, CAF-I 150 kDa subunit, CAF-I p150, CAF1, CAF1A, CAF1B, CAF1P150, CHAF1A, Chromatin assembly factor 1 subunit A, Chromatin Assembly Factor 1 Subunit A p150, Chromatin assembly factor I (150 kDa), Chromatin assembly factor I 150 kDa, Chromatin assembly factor I p150 subunit, DCAF1, hp150, MGC71229, P150,

Immunogen: Recombinant protein within human p150 CAF1 aa 100-300.

Conjugate: Unconjugated

Modification: Unmodified