Product Description

p21 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16025-50, BZ16025-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P38936

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 18 kDa; Observed MW: 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of p21 in mouse kidney tissue using p21 antibody, and DAPI., Immunofluorescence analysis of p21 in rat lung tissue using p21 antibody, and DAPI., Immunohistochemistry analysis of paraffin-embedded rat lung tissue using p21 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Negative control was used by secondary antibody only., Immunohistochemistry analysis of paraffin-embedded mouse liver tissue using p21 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Negative control was used by secondary antibody only.

Alternative Name: CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21

Immunogen: The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160

Conjugate: Unconjugated

Modification: Unmodified