Product Description

p27 Kip1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91000-50, BS91000-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46527(Human) Unigene:29897(Rat)

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100

Background: Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as "mitotic inhibitors." These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: p27 Kip1 polyclonal antibody detects endogenous levels of p27 Kip1 protein.

Bioworld Molecular Weight: 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining p27 KIP 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining p27 KIP 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDKN1B, AA408329, AI843786, Cdki1b, CDKN 1B, CDKN 4, CDKN1B, CDKN4, CDN1B, Cyclin Dependent Kinase Inhibitor 1B, Cyclin dependent kinase inhibitor p27, Cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, Cyclin-dependent kinase inhibitor p27 Kip1, KIP 1, KIP1, MEN1B, MEN4, OTTHUMP00000195098, OTTHUMP00000195099, p27, p27 Kip1, P27-like cyclin-dependent kinase inhibitor, p27Kip1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified