Product Description

p300 Polyclonal Antibody

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16469-50, BZ16469-100

Swiss-Prot: Q09472

Host: Rabbit

p300 Polyclonal Antibody-BZ16469

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: EP300: E1A binding protein p300. This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

Purification and Purity: Affinity Purified

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 264 kDa; Observed MW: 300 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of p300 in HELA, 293T, 823AVLysates using p300 antibody., Immunohistochemistry analysis of paraffin-embedded Human breastcancer using p300 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300

Immunogen: Synthesized peptide derived from the Internal region of human p300.

Modification: Unmodified

Conjugate: Unconjugated