p40-phox Polyclonal Antibody - BT-AP06789

p40-phox Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP06789-20, BT-AP06789-50, BT-AP06789-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of neutrophil cytosolic factor 4 was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Research Areas: Signal Transduction; Metabolism

Target Protein: NCF4

Applications: IHC-p, WB, ELISA

Reactivity: Human, Mouse

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: p40-phox Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 39031

UniProt Accession: Human: Q15080; Mouse: P97369

Synonyms: NCF4; SH3PXD4; Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox

GeneID: Human: 4689; Mouse: 17972

Research Use Only