p40-phox (R149) Polyclonal Antibody-BS1563

p40-phox (R149) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1563-50, BS1563-100

Sizes: 50µl, 100µl

Swiss-Prot: Q15080

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p40 phox (R149) polyclonal antibody detects endogenous levels of p40 phox protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p40-phox (R149) pAb at 1:500 dilution
Lane 1: THP-1 whole cell lysate (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: Jurkat whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p40-phox (R149) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 39 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40phox; NCF4; SH3PXD4

Immunogen: Synthetic peptide, corresponding to amino acids 115-170 of Human p40-phox.

Conjugate: Unconjugated

Modification: Unmodified