p44/42 MAPK (Erk1/2) Monoclonal Antibody - BT-MCA2695

p44/42 MAPK (Erk1/2) Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Number: BT-MCA2695-50, BT-MCA2695-100

Citations, Manuals and MSDS Available upon request.

Background: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

Research Areas: Others

Target Protein: p44/42 MAPK (Erk1/2)

Applications: WB, IHC, FCM

Reactivity: Human, Mouse

Clonality: Monoclonal

Clone ID: 3F8

Host: Mouse

Isotype: Mouse IgG2b

Full Product Name: p44/42 MAPK (Erk1/2)

Immunogen: Purified recombinant fragment of human MAPK expressed in E. Coli.

Storage: 4°C|-20°C for long term storage

Formulation: Ascitic fluid containing 0.03% sodium azide.

Molecular Weight (Da): 42kDa

UniProt Accession: P28482

Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, MAPK1

GeneID: 5594

Research Use Only