p47-phox (P366) Polyclonal Antibody-BS3261

p47-phox (P366) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3261-50, BS3261-100

Sizes: 50µl, 100µl

Swiss-Prot: P14598

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p47-phox (P366) polyclonal antibody detects endogenous levels of p47-phox protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p47-phox (P366) polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p47-phox (P366) pAb in paraffin-embedded human testis tissue.

Bioworld Molecular Weight: ~ 45 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A

Immunogen: Synthetic peptide, corresponding to amino acids 330-380 of Human p47-phox.

Conjugate: Unconjugated

Modification: Unmodified