p47-phox Polyclonal Antibody
Catalogue Numbers: BS61365-50, BS61365-100
Sizes: 50µl, 100µl
Alternative Name: Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P14598
Host: Rabbit
Reactivity: Human
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: NCF1 polyclonal antibody detects endogenous levels of NCF1 protein.
Bioworld Molecular Weight: ~ 47 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of NCF1 polyclonal antibody at 1:500 dilution
Lane 1: Jurkat whole cell lysate(40ug)
Lane 2: THP-1 whole cell lysate(40ug)
Immunogen: A synthetic peptide corresponding to residues in Human NCF1 around 296-341.
Conjugate: Unconjugated
Modification: Unmodified