Product Description

p53 (2D10) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3227-50, MB3227-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P04637

Host: Mouse

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 44 kDa; Observed MW: 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of p53 in Hela using p53 antibody and DAPI., Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using p53 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Western blot analysis of p53 in T47D and COS7 lysates using p53 antibody

Alternative Name: TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Immunogen: Synthetic Peptide of p53

Conjugate: Unconjugated

Modification: Unmodified