p53 Mutant Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91004-50, BS91004-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04637(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, IHC FC IP, ICC IF
All Applications: WB:1:500
IHC:1:100
FC:1:50-1:100
ICC:1:100-1:500
Background: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: p53 Mutant polyclonal antibody detects endogenous levels of p53 Mutant protein.
Bioworld Molecular Weight: 53 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Mutant p53 on 293 cell using anti-Mutant p53 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Mutant p53 antibody. Counter stained with hematoxylin.
Alternative Name: TP53, P53, Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein p53, Tumor suppressor p53,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified