Product Description

p53 (phospho-S15) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4144-50, BS4144-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: p53 plays a major role in the cellμlar response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at mμltiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15, the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumμlation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: Cellμlar tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human p53 around the phosphorylation site of Serine 15.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-p53 (S15) polyclonal antibody detects endogenous levels of p53 protein only when phosphorylated at Ser15

Molecular Weight: ~ 53 kDa

Pathway: Regμlation of Apoptosis, Hypoxia Signaling, Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04637

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only