Product Description

p53R2 (F156) Polyclonal Antibody

Catalogue Numbers: BS9220-50, BS9220-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7LG56

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: p53 is a transcription factor that mediates cell cycle arrest and apoptosis by binding to DNA and activating the transcription of specific genes. p53 is also thought to be involved in DNA repair by the transcriptional activation of a ribonucleotide reductase gene, p53R2, after exposure to genotoxic stresses. p53R2 displays a significant similarity to ribonucleotide reductase small subunit (R2), and the expression of R2 is elevated at the onset of the S-phase of the cell cycle. However, only p53R2 expression is induced in response to ultraviolet and g-irradiation and adriamycin treatment. p53R2 translocates to the nucleus upon DNA damage, and subsequently, supplies an immediate pool of dNTPs necessary for DNA repair.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p53R2 (F156) polyclonal antibody detects endogenous levels of p53R2 protein.

Extra Notes: Western blot (WB) analysis of RRM2B pAb at 1:2000 dilution
Lane1:A375 whole cell lysate(20ug)
Lane2:HCT116 whole cell lysate(20ug)
Lane3:The Brain tissue lysate of Mouse(40ug)
Lane4:The Prostate tissue lysate of Rat(40ug)
Lane5:CT26 whole cell lysate(40ug)
Lane6:H460 whole cell lysate(40ug)
Lane7:PC3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: RRM 2B; RRM2B; p53R2; p53 R2; MGC42116; MTDPS8A; MTDPS8B;

Immunogen: Synthetic peptide, corresponding to amino acids 123-178 of Human p53R2.

Conjugate: Unconjugated

Modification: Unmodified