p53RFP Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-15118-60, E-AB-15118-120, E-AB-15118-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: RNF144B
Target Synonym: bA528A10.3; E3 ubiquitin protein ligase RNF144B; E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain containing protein 2; IBR domain-containing protein 2; IBRDC 2; IBRDC2; KIAA0161; MGC71786; OTTHUMP00000016079; OTTHUMP00000016080; OTTHUMP00000039360; p53 inducible RING finger protein; p53-inducible RING finger protein; p53RFP; PIR2; R144B; Ring finger 144B; RING finger protein 144B; RNF 144B; RNF144B
Research Areas: Cell Biology
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse
Application: WB, IHC, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q7Z419
Background: p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.
Concentration: 0.4 mg/mL
Immunogen: Recombinant protein of human RNF144B
Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Purification Method: Affinity purification
Dilution: WB 1:200-1:1000, IHC 1:50-1:200
Calculated MW: 34kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only