p54nrb Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91006-50, BS91006-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15233(Human) Q99K48(Mouse) Q5FVM4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IF, IHC
All Applications: WB:1:500-1:2,000
ICC:1:50
IHC:1:50-1:200
Background: Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: p54nrb polyclonal antibody detects endogenous levels of p54nrb protein.
Bioworld Molecular Weight: 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution., ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: NonO protein, p54nrb, NONO, NRB54, 52 kDa subunit, 54 kDa nuclear RNA and DNA binding protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA binding p52/p100 complex 52 kDa subunit, DNA-binding p52/p100 complex, NMT 55, NMT55, Non Pou domain containing octamer (ATGCAAAT) binding protein, Non POU domain containing octamer binding, Non POU domain containing octamer binding protein, Non-POU domain-containing octamer-binding protein, Nono, NonO protein, NONO, NRB 54, NRB, NRB54, Nuclear RNA binding protein 54kD, P54, p54(nrb), p54nrb, PPP1R114, Protein phosphatase 1 regulatory subunit 114,
Immunogen: Recombinant protein of C-terminal human p54nrb.
Conjugate: Unconjugated
Modification: Unmodified