Product Description

p57 Kip2 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67001-50, MB67001-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregμlated in quiescent cells and in cells treated with cAMP or other negative cell cycle regμlators. Downregμlation of p27 can be induced by treatment with interleukin-2 or other mitogens, this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway .p57 Kip2 (Cyclin-dependent kinase inhibitor 1C) functions as a tumor suppressor. Mutations of p57 Kip2 have been associated with numerous human malignancies as well as Beckwith–Wiedemann syndrome (BWS), characterized by an increased risk of childhood cancer. The amino-terminal CDK inhibitory domain, common to the family, binds to and inhibits CDK/cyclin complexes and restricts cell cycle progression . The unique central region of p57 Kip2 interactes with LIMK-1, a downstream effector of the Rho family of GTPases. By sequestering LIMK-1 in the nucleus, p57 Kip2 disrupts actin dynamics within cells and may be linked to its essential role in embryonic development . In addition, the carboxyl-terminal QT domain of p57KIP2 binds to and inhibits JNK/SAPK activity regμlating cellμlar apoptosis and differentiation . Expression levels of human p57 Kip2 are more restricted then other CDK inhibitors and are controlled by ubiquitination/degradation via the Skp1/Cμl1/F-box-type E3 ubiquitin ligase complex SCF-Skp2. This effect is dependent on Thr310. A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regμlate protein stability .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: KIP2, Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p57 Kip2. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/4000)

Specificity: Recognizes endogenous levels of p57 Kip2 protein.

Molecular Weight: ~ 57 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P49918

Product: Mouse IgM. Supplied in crude ascites with 0.01% sodium azide.

Research Use Only