Product Description

p60 CAF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91007-50, BS91007-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13112(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IF, IP, IHC FC

All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50
FC:1:50-1:100

Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: p60 CAF1 polyclonal antibody detects endogenous levels of p60 CAF1 protein.

Bioworld Molecular Weight: 61 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SiHa
Lane 2: K562
Lane 3: A431, ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, CAF 1 subunit B, CAF 1A, CAF I 60 kDa subunit, CAF IP60, CAF-1, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CAF1, CAF1A, CAF1B, CAF1P60, CHAF 1B, CHAF1B, Chromatin assembly factor 1 subunit B, Chromatin assembly factor I, Chromatin assembly factor I p60 subunit, Human chromatin assembly factor-I p60 subunit, M phase phosphoprotein 7, M-phase phosphoprotein 7, MPHOSPH7, MPP 7, MPP7, p60 subunit,

Immunogen: Recombinant protein corresponding to the C-terminus of Human p60 CAF1.

Conjugate: Unconjugated

Modification: Unmodified