p73 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91010-50, BS91010-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O15350(Human) Q9JJP2(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents. The p73 isoform p73α inhibits drug-induced apoptosis in small cell lung carcinoma cells, while the p73 isoform p73β promotes it. p73α also prevents Bax activation, mitochondrial dysfunction, caspase activation and is able to reduce apoptosis induced by the BH3-only protein PUMA (p53 upregulated modulator of apoptosis). There is an equilibrium between p73α and p73β, demonstrated by the fact that p73α inhibits the pro-apoptotic effect of p73β.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: p73 polyclonal antibody detects endogenous levels of p73 protein.
Bioworld Molecular Weight: 70/60 kDa(12 isoforms)
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1,000 dilution., ICC staining p73 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TP73, P73, p53 like transcription factor, p53 related protein, p53-like transcription factor, p53-related protein, p73, P73, TP73, Tumor protein p73,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified