p95/NBS1 (Phospho-S343) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94088-50, BS94088-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60934(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. These phenotypes are similar to those of ataxia telangiectasia mutated (ATM) disease, where there is a deficiency in a protein kinase that is activated by DNA damage, indicating that the NBS1 (Nibrin) and ATM proteins may participate in common pathways. Nibrin is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. The phosphorylation of Nibrin requires catalytically active ATM. ATM physically interacts with and phosphorylates Nibrin on Serine 343 both in vitro and in vivo. Serine 343 is phosphorylated in vitro by ATM and the modification of this residue in vivo is essential for the cellular response to DNA damage. This response includes S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: p95/NBS1 (Phospho-S343) polyclonal antibody detects endogenous levels of p95/NBS1 protein only when phosphorylated at S343.
Bioworld Molecular Weight: 95 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Phospho-p95/NBS1(S343) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-p95/NBS1(S343) antibody. Counter stained with hematoxylin.
Alternative Name: NBN, NBS, NBS1, P95, AT V1, AT V2, At V, Cell cycle regulatory protein p95, FLJ10155, MGC87362, Nbn, NBN, NBS 1, NBS, NBS1, Nibrin, Nijmegen breakage syndrome 1 (nibrin), Nijmegen breakage syndrome, Nijmegen breakage syndrome protein 1, p95, p95 protein of the MRE11/RAD50 complex,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser343 of human p95/NBS1.
Conjugate: Unconjugated
Modification: Phosphorylation