PABPN1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91012-50, BS91012-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q86U42(Human) Q8CCS6(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, FC, IP,
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PABPN1 polyclonal antibody detects endogenous levels of PABPN1 protein.
Bioworld Molecular Weight: 49 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PABPN1 on MCF-7 cells lysates using anti-PABPN1 antibody at 1/500 dilution., ICC staining PABPN1 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PABP-2, Poly(A)-binding protein 2, PABII, PABPN1, Nuclear poly(A)-binding protein 1, OPMD, PAB2, PABII, PABP 2, pABP-2, PABP2, PABP2, PABPII, Pabpn1, poly(A) binding protein nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, PolyA binding protein II, Polyadenylate-binding nuclear protein 1, Polyadenylate-binding protein 2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified