PAK1/2/3 (Phospho-S144/S141/S139) Polyclonal Antibody-BS94089

PAK1/2/3 (Phospho-S144/S141/S139) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94089-50, BS94089-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75914(Human) Q13153(Human) Q13177(Human) O88643(Mouse) Q61036(Mouse) Q8CIN4(Mouse) P35465(Rat) Q62829(Rat) Q64303(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PAK1/2/3 (Phospho-S144/S141/S139) polyclonal antibody detects endogenous levels of PAK1/2/3 protein only when phosphorylated at S144/S141/S139.

Bioworld Molecular Weight: 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) on different lysates using anti-Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: SH-SY-5Y, ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.

Conjugate: Unconjugated

Modification: Phosphorylation