PAK2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91013-50, BS91013-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13177(Human) Q8CIN4(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:500-1:1,000
IHC:1:100-1:500
ICC:1:100-1:500
FC:1:50-1:100
Background: Three recently identified isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase STE20, involved in pheromone signaling. The α, β, and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PAK2 polyclonal antibody detects endogenous levels of PAK2 protein.
Bioworld Molecular Weight: 58 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PAK2 on mouse thymus tissue (1) and MCF-7 cell (2) lysate using anti-PAK2 antibody at 1/500 dilution., ICC staining PAK2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PAK-2, p27, p34, PAK2, C-t-PAK2, CB422, EC 2.7.11.1, Gamma PAK, Gamma-PAK, hPAK65, Kinase, p21 (CDKN1A) activated kinase 2, p21 (CDKN1A)-activated kinase 2a, p21 activated kinase 2, p21 protein (Cdc42/Rac)-activated kinase 2, p21 protein Cdc42 Rac activated kinase 2, p21-activated kinase 2, p21-activated kinase, 65-KD, p21-activated protein kinase I, p21CDKN1A activated kinase 2, p27, p34, p58, p65PAK, PAK 2, PAK-2, PAK-2p34, Pak2, PAK2, PAK65, PAKgamma, S6 H4 kinase, S6/H4 kinase, Serine threonine protein kinase PAK 2, Serine/threonine protein kinase PAK 2,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified