Product Description

PAK4/5/6 (phospho-S474) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4854-50, BS4854-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, ICC

Alternate Names: Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, PAK4, KIAA1142, Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6, PAK-6, PAK6, PAK5, Serine/threonine-protein kinase PAK 7, p21-activated kinase 5, p21-activated kinase 7, PAK-7, PAK7, KIAA1264

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Serine 474.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-PAK4/5/6 (S474) polyclonal antibody detects endogenous levels of PAK4, PAK5 and PAK6 only when phosphorylated at serine 474, 602, or 560, respectively. The antibody does not cross-react with phosphorylated PAK1, PAK2 or PAK3.

Molecular Weight: ~ 72 kDa (PAK4), 82 kDa (PAK6), 90 (PAK7)

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O96013/Q9NQU5/Q9P286

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only