Product Description

PAK4/5/6 (Phospho-Ser474) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65174-50, BS65174-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: p21 (RAC1) activated kinase 4(PAK4) Homo sapiens PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Mμltiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jμl 2008]

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-p, IF(paraffin, section), ELISA

Alternate Names: PAK4, KIAA1142, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: The antiserum was produced against synthesized peptide derived from human PAK4/5/6 around the phosphorylation site of Ser474. AA range:441-490

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity: Phospho-PAK4 (S474) Polyclonal Antibody detects endogenous levels of PAK4 protein only when phosphorylated at S474.

Molecular Weight: /

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O96013

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only