PAK4 (phospho Ser474) rabbit pAb - ES4457

PAK4 (phospho Ser474) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES4457-50, ES4457-100

Citations, Manuals and MSDS Available upon request.

Background: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Alternate Name: PAK4; KIAA1142; Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4

Source: Rabbit

Applications: WB; IHC; IF; ELISA

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Reactivity: Human; Mouse; Rat

Immunogen: The antiserum was produced against synthesized peptide derived from human PAK4/5/6 around the phosphorylation site of Ser474. AA range:441-490

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 64kD

Human Gene ID: 10298

Human SWISS Prot NO: O96013

Subcellular Location: Cytoplasm. Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.

Research Use Only