Product Description

Pan-Crotonyl-K Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS74126-50, BS74126-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Lysine crotonylation (Kcr) was first discovered about 10 years ago, as a PTM of histone. It was revealed that Kcr is an evolutionarily conserved and common PTM that occurs in both core histone and some non-histone proteins in a variety of organisms. Similar to other types of PTM, Kcr is a reversible modification. The classic histone acetyltransferases (HATs), p300/CBP, PCAF, and MOF, are responsible for most crotonylation events, while the histone deacetylases (HDACs) HDAC1/2/3 and SIRT1/2/3 reverse these reactions . The chromodomain protein CDYL acts as a crotonyl-CoA hydratase to negatively regμlate histone Kcr by reducing substrate supply. CDYL-regμlated Kcr of RPA1 plays an important role in homologous recombination (HR)-mediated DNA repair (23), while HDAC-regμlated histone crotonylation is reduced after DNA damage .

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Other Modified

Immunogen: A synthetic peptide corresponding to a sequence containing acetylatedK.

Dilution: WB, 1:500 - 1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Other Modified

Molecular Weight: 10-250KDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only