Pan-Crotonyl-K Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A20502-20, A20502-100
Citations, Manuals and MSDS Available upon request.
Background: Lysine crotonylation (Kcr) was first discovered about 10 years ago, as a PTM of histone. It was revealed that Kcr is an evolutionarily conserved and common PTM that occurs in both core histone and some non-histone proteins in a variety of organisms. Similar to other types of PTM, Kcr is a reversible modification. The classic histone acetyltransferases (HATs), p300/CBP, PCAF, and MOF, are responsible for most crotonylation events, while the histone deacetylases (HDACs) HDAC1/2/3 and SIRT1/2/3 reverse these reactions. The chromodomain protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone Kcr by reducing substrate supply. CDYL-regulated Kcr of RPA1 plays an important role in homologous recombination (HR) -mediated DNA repair (23), while HDAC-regulated histone crotonylation is reduced after DNA damage.
Category: Other Modified Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat
Observed Molecular Weight: 10-250kDa
Immunogen: Synthetic peptide
Species: Human
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: Pan-Crotonyl-K
Research Use Only