Pan-methyl-Histidine Polyclonal Antibody-BS74112

Pan-methyl-Histidine Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74112-50, BS74112-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, DB

All Applications: WB,1:500 - 1:2000 | DB,1:50 - 1:200

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Methylated

Bioworld Molecular Weight: 10-100KDa

Immunogen: A synthetic peptide of Methylated HistidineHis.

Conjugate: Unconjugated

Modification: Methylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using pan-methyl-Histidine antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 300s., Dot-blot analysis of all sorts using pan-methyl-Histidine pAb at 1:1000 dilution.
Peptide 1: Peptide library
Peptide 2: Peptide library
Peptide 3: Peptide library
Peptide 4: Peptide library
Peptide 5: Histidine methylation modified peptide library mix
Peptide 6: Control peptide library mix